FastQCFastQC Report
Thu 28 Apr 2016
ERR1041789.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041789.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8624749
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG173740.2014435434584821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170180.19731588710581605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159940.185443077821743No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA151800.1760051220041302No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT147400.17090352426488006No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC133900.15525089483763527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113150.13119222368094421No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC110320.12791096877138106No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG108200.1254529262242878No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG103440.11993392503364445No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC103340.11981797963047967No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT103230.11969043968699843No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC103120.11956289974351718No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG98530.11424100573825394No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG94090.1090930298377379No Hit
GATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTT88260.10233341283323143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100000.019.99851
TCTAATA29050.015.029262
TTAACGG33650.014.95393735
GGTAAAC26000.014.87115435
AATACTG34050.014.83265
TACCCCG18900.014.682545
TATTAGA40100.014.6708242
ATTAGAG39150.014.4125163
GTATTAG51250.014.222441
TTGGTAA27850.014.2154433
TACTGGT30550.014.1702137
GTATCAA142250.014.0326892
GATGCTA28950.013.99481914
GGGTAAG15150.013.9207931
TAATACT39650.013.8108454
ACCGTCC5500.013.790918
ACCAGTA27100.013.78966812
TAACGGC36500.013.73561636
ATGCTAG28750.013.70608815
CAGTATT26800.013.66791114
Produced by FastQC (version 0.11.5)