FastQCFastQC Report
Thu 28 Apr 2016
ERR1041788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4654345
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175180.3763794905620447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160400.3446242167265211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90880.1952584090779691No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG64030.13757037778677775No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC55850.11999540214573694No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG48790.10482678013769928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80750.017.7554191
TATACTG5650.016.0442495
CCGAGTT7550.015.6821213
GGGTAAG10150.014.0344831
ATTAGAG14350.013.7944253
TTAACGG13150.013.78707235
GTTATAC3900.013.7564113
GTCGCCC15350.013.73941337
GTATCAA104200.013.7240882
GCTATAC3800.013.6315793
CTACCCG3000.013.56666710
TAACGGC13650.013.28205236
GTAAGAT10500.013.2142873
AGGTCGC16100.013.21428635
TACACCG4450.012.8876415
TATTAGA15650.012.8849842
GTATTAG21750.012.6735631
TATACCG2204.094545E-912.6136365
TCTATAC4600.012.4673923
GTACTAG1501.9464316E-512.3333331
Produced by FastQC (version 0.11.5)