FastQCFastQC Report
Thu 28 Apr 2016
ERR1041787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073442
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT519350.6432820103247164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT517320.640767593301593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT357590.44292137108311425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160930.19933257710899516No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG134650.16678140500668734No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG112930.13987838148834167No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC111520.13813191449198495No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC100780.12482903822186374No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA98400.12188110102233968No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT98290.12174485182404234No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT97790.1211255372863272No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG95000.11766976216587673No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC89070.11032469174857515No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG87130.10792175134224039No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC82700.10243462453808426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175700.019.7635171
TTAACGG28400.016.35035335
TAACGGC28700.016.05052236
TCTAATA34650.015.6969712
ATTAGAG32650.015.2986213
AACGGCC30450.015.18883437
GGTAAAC28250.014.99646135
AATACTG36400.014.9931335
GTATTAG47000.014.9180851
TAATACT41800.014.7822964
TATTAGA36050.014.7794732
TTTAACG32650.014.73200634
CTCTAAT32000.014.6843761
ACCGCCT34150.014.03074612
TTGGTAA31150.014.0160533
ATGCTAG31200.013.99358815
GTGACAC34350.013.94905424
ATACTGG34700.013.9149876
GGGGTTA14300.013.8426576
GTATCAA253650.013.6899272
Produced by FastQC (version 0.11.5)