FastQCFastQC Report
Thu 28 Apr 2016
ERR1041786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4556067
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97730.2145051861616609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95380.20934722865137848No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG84210.1848304689110147No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA66180.14525686299169877No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC58510.1284221676283514No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG56040.12300082505371408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52860.11602112084831062No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC49970.10967793054843136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60350.018.3927081
TATTAGA15100.015.682122
ATTAGAG14150.015.5583043
CCGAGTT6450.015.48837213
CCCGTTA1651.6489139E-814.57575914
GCGTTAG908.2806515E-414.3888891
GTATTAG19950.014.187971
GTATCAA79600.013.8517592
TCGGACG2601.8189894E-1213.51923113
GTAAGAT9450.013.3121693
TTAACGG14350.013.27874635
TAACGGC14850.013.08080836
CGAACTA18850.012.95490624
CCGTTAT2001.4659236E-812.94999915
ACCGCCT17050.012.80351912
TCACGTA24050.012.76923125
AATACTG14800.012.755
TCTCGTC12300.012.63414612
TTAGAGG23300.012.6244634
GTGACAC16550.012.40785524
Produced by FastQC (version 0.11.5)