FastQCFastQC Report
Thu 28 Apr 2016
ERR1041785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110526
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169100.18560948072592076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164370.18041768389662682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117570.12904853133617092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136650.020.3073561
ATTAGAG22550.014.6851443
TATACAC18950.014.64383
GTATCAA194250.014.2476192
TATTAGA24050.014.0769232
ATACACA26750.013.8317754
TTAACGG19350.013.28940635
TACACAT25800.013.1937995
TCTAATA18450.013.0352312
GTATTAG33250.012.7969921
ATCTCGT25250.012.6019837
AATACTG24000.012.4104165
TTTAGCG5700.012.33333426
TAATACT25950.012.2620424
CTCTAAT17700.012.1242931
ACGAGAC26150.012.09751522
TTTAACG22550.012.05986734
TAACGGC22150.011.69300336
GGTAAAC16200.011.64814935
TACCCCG15600.011.6217965
Produced by FastQC (version 0.11.5)