FastQCFastQC Report
Thu 28 Apr 2016
ERR1041784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13126273
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT473660.3608488106258341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT439620.33491608775773596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT352280.26837777943518315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175280.13353371516804505No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG132660.10106448342191267No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC132350.10082831585172729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173850.019.3459871
TTAACGG30750.016.30406635
TAACGGC32050.015.81591336
CTCTAAT41050.014.2862361
TTTAACG37750.014.06490134
TCTAATA46550.013.9495162
AACGGCC37450.013.58477937
TAATACT58700.013.457414
GGTAAAC40750.013.12024535
GTATCAA257800.013.0318082
AATACTG53000.012.9849055
ATTAGAG45400.012.7544053
GTATTAG60850.012.70831
CTAATAC58200.012.682993
TATTAGA50000.012.5062
ATACTGG51900.012.4046246
ATGCTAG43700.012.36155615
TACTGGT49550.012.3582247
GTTTAAC42250.012.21656833
GATGCTA46250.011.9614
Produced by FastQC (version 0.11.5)