FastQCFastQC Report
Thu 28 Apr 2016
ERR1041783.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041783.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758483
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT589191.0231687755264711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT428580.7442585139176412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT317180.5508047866078618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223040.3873242310518239No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG69950.12147296432063791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66000.11461351887293927No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63820.11082779961319675No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT62540.10860499197444883No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG60540.10513185503890521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA13450.017.33085419
ATCTCGT12300.017.14634137
ATCTCCG14100.017.05673810
TAACGGC14100.015.87588836
TTAACGG13900.015.83812835
CGAGCCC14950.015.59197415
ATTAGAG17000.015.3441163
GCCCACG15450.015.32686218
GGTATCA232650.015.2834741
CGGTCTA855.368794E-415.23529320
TATTAGA18550.014.66037752
TACACAT18650.014.48257355
TCTCCGA16650.014.11111111
TAGGCAT16300.013.73312929
CACGAGA17400.013.71551821
TCGTATG4050.013.70370437
ACGAGAC16750.013.58507422
GTATTAG28550.013.5429091
GGCACCG16650.013.4444459
ACCGCCT17350.013.32852912
Produced by FastQC (version 0.11.5)