FastQCFastQC Report
Thu 28 Apr 2016
ERR1041782.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041782.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15311862
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG163650.10687792248911335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.021.319281
ATTAGAG46600.014.3315453
TATTAGA52600.013.7870722
GTATCAA177750.013.5094222
GTATTAG70150.013.13328551
TTAACGG43800.012.79794535
TCTAATA39950.012.3178972
GTCGGGA34850.012.2625532
GGTAAAC30800.012.25324635
AATACTG44550.012.1257015
TTTAGCG14050.012.11387926
TAATACT51100.011.9109584
TAACGGC47350.011.87750836
TTGGTAA33800.011.71301833
TTTAACG51550.011.69932134
TCTTATA50150.011.69391837
CCTCTAG49250.011.64467051
TACCCCG27550.011.617065
ATACTGG46150.011.5850496
GGGGTTA18450.011.5311656
Produced by FastQC (version 0.11.5)