FastQCFastQC Report
Thu 28 Apr 2016
ERR1041781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13572938
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT433280.3192234430010658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT419490.30906352036677687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327330.24116370383479246No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG158300.11662913364814603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151930.11193597141606335No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC147500.10867212389830412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213650.016.7119121
TTAACGG37400.014.69117535
TAACGGC37800.014.53571436
TATTAGA48350.014.4250262
ATTAGAG43050.014.0952383
TTTAACG41700.013.66426934
TCTAATA46750.013.533692
CTCTAAT42950.013.4388831
AATACTG51350.013.3300885
GTATTAG64200.013.197821
TAATACT54700.013.1224864
GGTAAAC40400.013.0049535
TACCCCG21350.012.7377045
GTTTAAC45100.012.59312633
ATGCTAG43900.012.22095615
ATACTGG52050.012.1911626
TACTGGT48750.012.105647
CTAATAC63500.012.0614173
AACGGCC45500.012.03516537
TTGGTAA44150.012.02604733
Produced by FastQC (version 0.11.5)