FastQCFastQC Report
Thu 28 Apr 2016
ERR1041780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237841
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158780.7095231520023093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147710.6600558305974374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116130.5189376725156076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.249973076728865No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC24430.10916772013740028No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG23600.10545878818021476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.016.756391
GCGAACA1554.0199666E-1016.70967729
ATTAGAG7600.016.3092123
TATTAGA8450.015.9822472
ACTTACG700.00259352215.85714316
ACTACGC700.00259352215.8571438
CCGAGTT2150.015.48837213
TTAACGG7300.015.45890535
TAACGGC7400.015.2536
TAGGACC5450.014.9357794
CCGTTAT750.004106021514.815
GTATTAG10700.014.6962611
GGGGTTA3800.014.6052646
CGTATTA1406.0016646E-714.53571515
TTACGGG1152.2115832E-514.47826129
TTTAACG8100.014.38888934
GCACCGC7850.014.37579610
TATACTG2600.014.2307695
GGCACCG8000.014.1062519
CTACCCG2101.364242E-1014.09523810
Produced by FastQC (version 0.11.5)