FastQCFastQC Report
Thu 28 Apr 2016
ERR1041779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142533
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107740.3428444506390227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91910.29247107349389806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76170.24238408952268758No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC39970.12719039068165713No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG38830.12356274381207771No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG38270.12178074184105625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.12101702671061848No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT35170.1119160880729017No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG34880.11099326562362273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.022.7173231
TTAACGG10100.015.56930735
TCGCTAA1101.45338345E-515.13636414
TAACGGC10300.015.08737936
ATTAGAG11850.014.5189873
GCACCGC11850.014.36286910
GGCACCG11750.014.327669
ACCGCCT11650.014.13304712
TATTAGA13800.014.0760862
CTACCCG2950.013.7966110
GTATTAG17200.013.7674431
GTATCAA54300.013.6961332
TAGACCG950.001245915113.63157755
TAGTACG2202.8558134E-1013.4545454
TTTAACG12150.013.24691334
AACGGCC12000.013.10416737
TCTAGCG850.00940947513.05882428
TCTAATA12750.012.9137252
ATAGGCG4500.012.74444532
GTCTTAC3200.012.718751
Produced by FastQC (version 0.11.5)