FastQCFastQC Report
Thu 28 Apr 2016
ERR1041778.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041778.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774127
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89520.322696113047456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80420.2898930005727928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63770.22987411895706286No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.11866796293032006No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC29890.1077456078975476No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG28620.10316759110163307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC400.001931871623.1253
GGTATCA34250.018.6350361
TAACGGC6750.016.99259236
TTAACGG7000.016.6535
CCCGTTA1302.5926238E-715.65384614
CTACCCG2650.014.660377510
GGTAAAC7250.014.28965635
CGCAGTA2750.014.12727418
GTATAGA3150.014.0952391
AACGGCC8050.014.01863437
TAAGCGC800.00630158113.87523
CGTCTTA950.001245850113.63157910
TAGGACC6450.013.480624
TTTAACG8400.013.43452434
ATTAGAG9650.013.036273
TAATACT12150.012.9423884
TATTAGA11300.012.9336282
AATACTG10750.012.9069775
CTCTAAT9200.012.8695651
TCTAATA10200.012.6960782
Produced by FastQC (version 0.11.5)