FastQCFastQC Report
Thu 28 Apr 2016
ERR1041777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030811
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT579861.9132172873861153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT444051.465119402034637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264020.8711199741587317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258860.8540948280839683No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT118070.3895656971021948No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65730.21687264563841163No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60970.20116727832913367No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60430.1993855769957282No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55710.1838121875629988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45480.1500588456357061No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA39340.12980024158550302No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA37880.1249830490914808No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.11960495062212721No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT31490.10389958331284926RNA PCR Primer, Index 23 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.10337167180665505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT6400.022.54687737
TACACAT27700.021.57225
GCCGTCT6900.020.64492636
ATACACA30400.020.2648034
TATACAC29850.020.0184253
ATCTCCG28850.019.6863110
ACACATC30350.019.566726
ACATCTC32650.018.3016838
CTAGTAC2950.018.1864413
GTCGTCA2150.018.06976724
GCCGAGT2300.017.69565212
TATACTG4400.017.659095
CCGTATA1900.017.5263162
ATCTCGT28750.017.5026137
TCTCCGA32650.017.28177611
CACATCT33700.017.1824917
ACGAGAC32150.016.97511722
CCCACGA33000.016.8742419
CGAGCCC33950.016.72901315
GTATTAG11400.016.5526311
Produced by FastQC (version 0.11.5)