FastQCFastQC Report
Thu 28 Apr 2016
ERR1041776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7609463
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT408570.5369235647771728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT402530.5289860795696095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255140.335293042360545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169650.22294608699720336No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT117080.15386105432144162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142800.022.2958681
TTAACGG10650.016.84976635
TAACGGC11450.015.6724936
ATCTCCG32250.015.60310110
GTATCAA205050.015.5091442
TACACAT37400.015.4826195
CGTATTA4450.014.96629315
ACACATC37600.014.51462656
GTATTAG23350.014.498931
ACGAGAC34850.014.33285522
ATCTCGT32300.014.31888637
TATACAC40550.014.1430323
CTAATAC24550.014.0916513
TATTAGA21300.014.0704222
CCCACGA35350.014.02545919
ATTAGAG17200.013.9825583
TTTAACG14050.013.82562334
TAATACT23900.013.7008364
TTACTCG5950.013.68067219
ATACACA47300.013.6501054
Produced by FastQC (version 0.11.5)