FastQCFastQC Report
Thu 28 Apr 2016
ERR1041775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9900488
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547440.5529424408170588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT502250.5072982261076424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT330980.3343067533640766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212950.2150904076647535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204400.019.5046481
TTAACGG18200.018.29670335
TAACGGC18400.018.09782636
ATTAGAG25400.016.8248023
GTATTAG36700.016.18121
CTCTAAT23650.015.644821
TATTAGA32200.015.6273282
CTAATAC38200.015.5458113
AACGGCC21350.015.51053837
TTTAACG22100.015.40271434
TAATACT40100.015.2244394
TTAGACT11050.014.9004524
TCTATAC16600.014.8222893
AGGTCGC17600.014.29545435
TATACTG18000.014.2861115
AATACTG37250.014.2040285
GTATCAA284400.014.0050972
GGCACCG26300.013.9277569
TTAGAGG35050.013.7760344
TCTAATA30850.013.7325762
Produced by FastQC (version 0.11.5)