FastQCFastQC Report
Thu 28 Apr 2016
ERR1041774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8733890
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT478730.5481291841321565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365590.41858782283724666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263840.3020876150260651No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176840.20247564372805246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157750.018.43551
GTATTAG26850.015.77839851
TAGACCG1659.767973E-1015.696975
GACGGAC22850.015.54485726
CGAGACG23700.015.14345923
ATCTCGT22750.014.837
TATCTCG23150.014.6241936
CTTATAC117850.014.50487937
ACGAGAC25800.014.12596922
ACGGACT25300.014.11264727
CGCGATA1051.6576058E-414.09523914
TATACCG4900.013.9693875
ATTAGAG20000.013.8753
CCCACGA26650.013.67542219
ATCTCCG27650.013.58227810
TAATACT28450.013.5254844
CTAATAC26850.013.4357543
TACACAT34250.013.395625
TAGACTA7600.013.3881585
TATTAGA24450.013.3169732
Produced by FastQC (version 0.11.5)