FastQCFastQC Report
Thu 28 Apr 2016
ERR1041773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282313
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT552830.5376514019754116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT413090.40174812807196203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287750.2798494852276915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212760.2069184238993697No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG133790.13011663815330266No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT131560.12794786542677702No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC108700.10571551362033035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204700.017.1624321
ACGAGAC33400.016.3952122
CTCTAAT38600.016.3911911
CCCACGA33650.016.3283819
GGCACCG35200.016.18759
GCACCGC34450.016.16400510
ATTAGAG38000.016.065793
ATACTGG43800.015.9657536
GTATTAG52250.015.9330151
ATCTCGT32750.015.873281537
TTAACGG29600.015.812535
ATCTCCG35400.015.78248510
CGAGCCC35300.015.66997115
GGTAAAC36000.015.57083335
TAACGGC30000.015.54000136
TCTCCGA35800.015.39944211
TACACAT44800.015.3616085
CGAGACT36550.015.33652623
AGTACCG17900.015.296095
TAATACT57850.015.1901464
Produced by FastQC (version 0.11.5)