FastQCFastQC Report
Thu 28 Apr 2016
ERR1041772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13500722
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT462020.34221873467211605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT433420.32103468244142797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291230.2157143892008146No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160170.11863809950312287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169450.021.5624071
TAACGGC23100.018.17965336
TTAACGG23400.017.78846235
TTTAACG28200.015.54787334
AACGGCC27450.015.50091137
GTATTAG46400.015.4698281
GTATCAA238150.015.2956112
ATTAGAG36700.015.1730233
TATTAGA44750.014.5932962
TAATACT51400.014.3968884
TATACTG30550.014.3518825
CTAATAC46450.014.2185143
CTCTAAT34400.013.9287811
CTACACT26150.013.8661574
TTAGAGG47850.013.8025084
GGCACCG34750.013.5223039
TCTATAC23450.013.2537323
AGGTCGC23650.013.21987335
TCTAATA42400.013.1332552
ACCGCCT37850.013.00132112
Produced by FastQC (version 0.11.5)