FastQCFastQC Report
Thu 28 Apr 2016
ERR1041771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10335075
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT492390.476426150753623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415780.4022999349303222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT269640.26089796155325434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161540.1563026876921551No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT107730.10423726968599646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161100.019.7976421
TTAACGG18500.016.335
TAACGGC19050.016.02362336
GTATTAG36200.015.7403321
TAATACT39450.015.5690764
TATTAGA32550.015.4024572
GACCGTA29150.015.10463126
CTCTAAT29400.015.0391151
AATACTG37700.015.0159165
ACCGTAC29950.014.94824627
ATTAGAG27650.014.8535253
ATCTCCG32500.014.74307610
CTAATAC40200.014.5883083
TTTAACG21600.014.56018534
ACGAGAC32350.014.52550222
ATCTCGT30450.014.4597737
ACTAGAT30900.014.30906132
TCTATAC15050.014.2591353
CCCACGA32400.014.21759219
TACACAT42000.014.1392855
Produced by FastQC (version 0.11.5)