FastQCFastQC Report
Thu 28 Apr 2016
ERR1041770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10724970
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT632670.5899037479825118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT594350.5541740443096811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT345540.3221827193922221No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282390.263301435808212No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT228390.21295164461998495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT76900.019.8712625
ATCTCCG70900.019.72637410
GCGAATC65000.019.29692333
ACGAGAC71950.019.15566422
CCCACGA73600.018.8016319
CGAGCCC73600.018.70108815
GGCGAAT68300.018.68960632
CGAATCT66650.018.62490734
GGTATCA273900.018.3649141
TCTCCGA75950.018.34167311
ACACATC81350.018.30676
GCCCACG75800.018.25593818
ATCTCGT49600.018.12701637
GACTAAG76500.018.08888826
TAAGGCG75950.018.04937429
AGGCGAA75900.017.81752431
ACTAAGG79100.017.56447627
CACGAGA79150.017.5533821
ACATCTC83450.017.5134818
TATACAC87750.017.2455833
Produced by FastQC (version 0.11.5)