FastQCFastQC Report
Thu 28 Apr 2016
ERR1041769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8530213
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215380.25249076429861717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185960.2180015903471578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127830.14985557804945784No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT92240.10813329045828046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.025.8307821
GTATCAA92800.016.3669192
TACACAT36650.015.3956355
TTACTCG8700.015.09770219
GTATTAG31800.015.0094351
TTAACGG15950.014.96238335
TAACGGC16600.014.59939836
ATTAGAG21850.014.5629293
CTCTAAT22050.014.430841
TATACAC36300.014.3209363
TATTAGA27700.014.2924182
TAATACT33150.014.2865764
TTTAACG18450.014.23848234
ATACACA44250.014.0892654
TATACCG4100.013.9878045
ACGAGAC30900.013.83009622
CTAATAC31900.013.7445143
TTAACGC1356.578346E-613.70370335
CGTATTA7750.013.60645115
ATCTCGT29000.013.39655237
Produced by FastQC (version 0.11.5)