FastQCFastQC Report
Thu 28 Apr 2016
ERR1041768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13361302
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT301730.2258238007044523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277650.20780160496334862No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT214570.16059063705019166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190020.14221667918291198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86050.025.669961
GTATCAA128550.017.082462
TACACAT66450.015.8690755
ATCTCCG58200.015.607388510
TATACAC67200.015.3891363
ATCTCGT56600.015.13339237
ACACATC67900.014.4948456
ACGAGAC61600.014.38555122
TCTCCGA62400.014.34935811
ATACACA85350.014.0456944
CCCACGA64850.013.7787219
GTATTAG33950.013.6229751
TAATACT40500.013.5666664
TCTTATA138250.013.50198937
CACGAGA65900.013.47496221
CGAGCCC67600.013.32766315
TATACCG5600.013.2142855
CTAATAC35900.013.19223
CATCTCG66600.013.02777836
GCCCACG69750.012.99641618
Produced by FastQC (version 0.11.5)