FastQCFastQC Report
Thu 28 Apr 2016
ERR1041767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18459638
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT412410.22341174837773092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT408150.22110401081538003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT268120.1452466185956626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140250.022.2527621
TTAACGG28600.017.1416135
TAACGGC29300.016.54266236
GTATTAG65800.015.8009111
GTATCAA203550.015.2507982
TTTAACG34400.014.62790734
TAATACT77350.014.39819054
CTAATAC68750.014.181093
TCGCTAA9350.014.04812814
AACGGCC35050.013.82881637
TATACTG42350.013.7166475
GCACCGC40050.013.67290910
ACGAGAC44150.013.61834722
CTCGCTA9800.013.59183813
TATTAGA60250.013.54107952
TAGGACC32400.013.1898154
CTAATCG9400.013.1861726
TACCCCG22200.013.1666675
TCTATAC38700.013.1459953
ATCTCGT41550.013.09025337
Produced by FastQC (version 0.11.5)