FastQCFastQC Report
Thu 28 Apr 2016
ERR1041766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13813627
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264240.19128936954791093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT259750.1880389560250903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169380.12261804955353145No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT159660.11558151961103336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.022.6459921
TATACCG4350.015.7356335
CGAGACG47000.015.70531923
TACACAT60100.015.5141445
GTATCAA124900.015.4339482
GTATTAG32850.015.374431
TATCTCG46750.015.1561536
ATCTCCG50050.015.15484610
GACGGAC47650.015.02518326
ACGAGAC50400.014.75595222
ATCTCGT47200.014.54131337
ACGGACT48750.014.53435927
TATACAC64400.014.2197213
CGGACTC51300.014.06432728
TCTCCGA54400.013.94301411
ATACACA76900.013.7847864
TCTATAC22950.013.7843133
ACACATC59650.013.7703276
CCCACGA54700.013.76508319
TCTTATA144900.013.64837837
Produced by FastQC (version 0.11.5)