FastQCFastQC Report
Thu 28 Apr 2016
ERR1041765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11033851
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262850.23822145142253598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT251240.22769928649571214No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT184010.1667686105241044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158440.14359447123221078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80550.021.9335821
TACACAT57700.016.6724435
TATACAC59600.015.6753353
ACACATC58350.015.472156
GTATCAA115000.015.3630452
ATCTCCG55400.015.09386310
ACGAGAC56050.014.68777822
TATACTG19500.014.5153855
ATCTCGT53850.014.49767937
CCCACGA57150.014.46981619
ATACACA71500.014.3860134
TCTCCGA58200.014.36769711
CACGAGA58700.014.05621721
CGAGACT57400.014.05226523
TCTTATA137850.013.89009937
CTAATAC27500.013.7909093
ACATCTC69300.013.72158
TAATACT30450.013.66995054
GCCCACG60650.013.6347918
CGAGCCC61400.013.55863215
Produced by FastQC (version 0.11.5)