FastQCFastQC Report
Thu 28 Apr 2016
ERR1041764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9877796
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT585280.5925208416938353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT459470.465154372493621No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT427950.43324442011153097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263640.2669016448608576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186940.18925274423565744No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT118610.1200773937829856TruSeq Adapter, Index 11 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT86250.023.0579725
TATACAC87600.022.322493
ACACATC92800.021.1912736
CCGTCTT24600.020.831337
ATACACA98900.020.6511634
GCCGTCT25300.020.40118636
ATCTCCG93050.020.37882810
ACATCTC99600.019.7444788
CACATCT103850.018.437657
TTATACA105800.018.2202282
GACCGTA101800.017.82760426
ACCGTAC101800.017.79125827
TCTCCGA106300.017.68203411
ATCTCGT102200.017.61301437
CGTACTA103000.017.5660229
GTACTAG103400.017.5338530
ACTAGAT105200.017.3921132
ACGAGAC107850.017.35929522
AGACCGT105300.017.32288625
CTAGATC105800.017.2934833
Produced by FastQC (version 0.11.5)