FastQCFastQC Report
Thu 28 Apr 2016
ERR1041763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857093
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1365463.540127240903966No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT944202.4479575680441203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT819712.125201544271813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478231.2398715820437827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT428701.1114588110787063No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA269230.6980127261644975No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT165050.42791293857835416RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG145440.3770715406654701No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC140710.3648084191903073No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135020.3500563766546464No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT115920.30053721805515193No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC107300.27818878103276223No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA92070.2387030854584009No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA89980.23328449689960806No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA83020.21523981921099644No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG79820.2069434156760026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75620.1960543860363232No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA71860.18630611188270543No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA67850.17590968120291628No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62970.16325766581205067No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.1618317214544736No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA58960.15286123513226152No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA56530.1465611536978756No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA54680.14176479540420725No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG54380.14098700757280158No Hit
AAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAA49450.1282053608767017No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCCT48190.12493865198479788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47100.1221126895306906No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47070.12203491074755005No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA46420.1203497037795044No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA44910.11643483836142919No Hit
GTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA44570.11555334548583608No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44320.11490518895966471No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42780.1109125447584489No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA41550.10772361464968566No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41130.10663471168571773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG303.6010367E-430.8333345
TATACAC172800.029.366613
CCGTCTT21550.029.35962737
TACACAT178850.028.3732175
ATACACA184800.027.499734
GCCGTCT22750.027.48571436
TTATACA187600.026.9315012
ACACATC201700.025.1680726
CTTATAC211900.023.6772081
CACATCT214750.023.6300357
ACATCTC217100.023.5617248
CGTTATA1005.347829E-1022.22
CATCTCC241000.021.1790459
ATCTCCG240800.021.1198110
ATCTCGT190950.019.54150237
TCTCCGA264000.019.26382611
AATCTCG218550.019.23221236
GCGAATC247550.019.04928433
CCCACGA266000.019.03552619
CGAATCT245200.019.0281434
Produced by FastQC (version 0.11.5)