FastQCFastQC Report
Thu 28 Apr 2016
ERR1041762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342599
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153731.145017983776243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112850.840533919658811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91250.6796519288335534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78810.5869958193027106No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA23750.1768957075046235No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT23700.17652329548882428No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA20450.1523165144618758No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.14792205267544514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.137196586620428No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.11567117210723381No Hit
CAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15260.1136601472219181No Hit
AAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAA15010.11179808714292204No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG14680.10934016783864728No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.10554156527749538No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA13790.10271123395742139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA450.003826137620.5555577
ACCGTCG500.007035483618.523
GCGTCGG609.2379167E-418.59
TTAACGG2100.017.61904735
TAACGGC2250.016.44444536
GACCGTG803.3830965E-416.18757
TAATACT4500.016.0333334
CGAACTA2900.015.94827624
TTATGCG1751.3096724E-1015.8571434
GTAAGAC1403.474088E-815.8571423
TATACGC700.002592798315.8571425
TAAGACT4550.015.8571424
TCGCGCC700.002592798315.8571429
CTCTAAT2700.015.7592591
TATGCGG1954.1836756E-1115.1794875
ATTAGAG3700.015.03
TCTAATA4650.014.7204312
GTATTAG4050.014.6172841
GGTAAAC2550.014.50980435
AACGGCC2550.014.50980437
Produced by FastQC (version 0.11.5)