FastQCFastQC Report
Thu 28 Apr 2016
ERR1041761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4876190
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225630.46271781862478695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210560.43181254216919357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT152710.31317483527098No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84470.17322950910444423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.020.703421
TTAACGG7200.017.72916635
TAACGGC8000.015.95625136
GTATTAG17200.015.8110471
TTTAACG8700.015.52298734
ATTAGAG12550.015.1832673
GTATCAA120300.014.6093112
TTACTCG3800.014.60526419
CGTATTA3700.014.515
TCTATAC7550.014.211923
TATTAGA15500.014.2032252
TAATACT17000.014.1470594
TTAGAGG16750.013.805974
CTACGAC3050.013.3442623
TACTGGT13000.013.2346157
TACTCCG8250.013.2303045
ATCTCCG13650.013.1465210
TATACTG10150.013.1231535
ATCTCGT13000.013.09230737
AACGGCC9500.013.04736837
Produced by FastQC (version 0.11.5)