FastQCFastQC Report
Thu 28 Apr 2016
ERR1041760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6513013
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285540.43841460165978485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260700.4002755713830143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187010.287132852337313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107120.1644707295993421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110700.019.3857271
TTAACGG9950.018.22110635
TAACGGC10000.018.1336
TATACCG3200.016.7656255
TTTAACG11900.016.01260434
TAATACT24250.015.6391744
GTATTAG22350.015.4787461
TATACTG14850.015.3232325
ATTAGAG15800.015.22151853
TATTAGA20550.015.2141112
CTCTAAT16550.015.0906341
TCTATAC10900.014.9357813
AACGGCC12250.014.837
CGAACTA10450.014.6937829
GGCACCG14400.014.3888889
CTAATAC24700.014.3056683
ACGAGAC16950.014.29793522
ATCTCGT15250.014.19344237
GGTAAAC14450.014.08304535
TAGACTG10450.013.9856455
Produced by FastQC (version 0.11.5)