FastQCFastQC Report
Thu 28 Apr 2016
ERR1041759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8711787
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325580.37372355407679275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293630.3370491037028339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214800.24656250204464367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118770.13633253430094194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125300.021.0099751
TTAACGG18800.018.89361835
TAACGGC19250.018.35584436
GTATTAG38450.017.3211981
TTTAACG22200.016.66666634
TATTAGA33400.016.395212
ATTAGAG27850.016.3411123
AACGGCC22250.016.04719237
TAATACT39750.015.7773584
CTAATAC37800.015.3677253
CGAACTA20800.015.12019329
TATACTG17050.014.8651035
GGCACCG28450.014.6959589
CTCTAAT26000.014.6576931
ACCGCCT27550.014.63883912
AATACTG35850.014.5523015
GCACCGC27850.014.48114910
CGTATTA9350.014.44385115
TTAGAGG37150.014.3916554
GTATCAA183050.014.3512692
Produced by FastQC (version 0.11.5)