FastQCFastQC Report
Thu 28 Apr 2016
ERR1041758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7336546
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270410.36857943778993546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT258630.35252283567771536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186250.253866056315874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.11426357852864276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.026.0837821
TAACGGC11150.018.5829636
TTAACGG11250.018.41777635
GTATCAA131950.016.5862062
AACGGCC12550.016.5099637
GTATTAG26950.016.4749531
TTTAACG13200.016.39772834
ACCGCCT16100.015.28260912
TTTAGCG4800.015.03125126
GGCACCG15800.014.8702539
TTACTCG7200.014.38888919
TCGCTAA2850.014.28070314
GCACCGC16550.014.19637510
ATTTCGG7300.013.93835629
TATACTG17200.013.8755
TATTAGA27050.013.6783742
ATTAGAG21950.013.6537593
TAATACT28050.013.6524074
AATTTCG7700.013.45454528
CTAATAC24500.013.4408163
Produced by FastQC (version 0.11.5)