FastQCFastQC Report
Thu 28 Apr 2016
ERR1041756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645914
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT42250.6541118477072799No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT12720.19693024148725682TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9150.14165972559814466No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG9020.13964707375904534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.12555851088535006No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC7970.12339103967401234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC5400.027.0648163
TTATACA5900.025.0847432
TACACAT6450.024.0930235
GGTATCA7750.022.9161281
CTTATAC6400.022.5468751
ACACATC7150.021.2167846
ACATCTC7500.021.2133338
GGCTTAG705.0962135E-621.1428591
ATACACA7350.021.1428594
GGTATAT450.003824020320.5555571
GTCGTGC759.255466E-619.7333348
CACATCT7750.019.3354847
ATCTCCG7900.018.96835310
TATAAAC701.2181482E-418.53
GATATAA500.007031628418.4999981
GTACTAG8250.017.93939430
CGTACTA8300.017.83132629
GACCGTA8350.017.7245526
TACTAGA8250.017.71515331
GTGTAGG1054.789217E-717.6190471
Produced by FastQC (version 0.11.5)