FastQCFastQC Report
Thu 28 Apr 2016
ERR1041754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305695
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT334750.4030367115575518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310200.37347867938805845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225010.27091050177017095No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT151340.1822123253984164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118460.14262503017507866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110200.021.2699621
ATCTCCG36550.017.1080710
ACGAGAC37250.016.43892722
ATCTCGT36900.015.99322537
TACACAT43400.015.9423955
CCCACGA38550.015.78858719
TATACCG4750.015.5789475
TCTCCGA39450.015.52218111
ACACATC43000.015.0581396
GCCCACG41200.014.99757218
CACGAGA40750.014.98159521
CGAGCCC41750.014.88862215
CATCTCG40900.014.74572236
GTATCAA159250.014.7302992
TAGACAG13250.014.3811335
CGAGACC43300.014.09930723
GTATTAG21450.013.9720291
CTCTCTA43250.013.8589629
GACCTCT44550.013.786756526
CCGAGCC46550.013.67132114
Produced by FastQC (version 0.11.5)