FastQCFastQC Report
Thu 28 Apr 2016
ERR1041753.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041753.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991714
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT352881.7717403201463664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT228011.1447928768889508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215691.08293660636015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123390.6195166575120725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114660.5756850632169077No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT72040.3616985169557477TruSeq Adapter, Index 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64650.32459479624082577No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC62500.31380007370536134No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG62120.3118921692572327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.2218189961008458No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC44040.22111608393574578No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.17869031397078095No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.14289200156247334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.13967868880773043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24380.12240713275098733No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23350.117235707536323No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.11527759507640152No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG22150.11121074612118005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT56200.026.0711735
TATACAC58250.024.8042933
ATACACA61750.023.6979774
ACACATC62150.023.6645226
CACATCT65850.022.4191327
AATCTCG2150.022.3720936
CCGTCTT10550.022.27014437
ACATCTC66850.022.1944668
TATACCG1550.021.483875
GCCGTCT11200.021.47321336
TTATACA67400.021.3820462
ATCTCCG69150.021.2689810
TCTCCGA75800.019.40303611
CATCTCC77350.019.181649
GACTAGG75950.019.12113226
CACGAGA76500.019.10457621
CGAGACT76700.019.10299923
CCCACGA76550.019.09209819
ACGAGAC76450.019.04447422
ATCTCGT70450.019.03832437
Produced by FastQC (version 0.11.5)