FastQCFastQC Report
Thu 28 Apr 2016
ERR1041752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9004658
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT322950.3586477132168706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316380.35135148941803235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT229060.2543794556106406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122330.1358519113107905No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT99650.11066494696411568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123650.020.0335621
CGAGACG27500.017.01999923
AATCTCG9800.016.9897966
TATCTCG27350.016.57221236
GACGGAC28050.016.48841326
ACGGACT28100.016.32740227
ACGAGAC30850.015.71150722
CCCACGA30300.015.6914219
ATCTCCG31150.015.61958110
TCTCCGA31650.015.37282811
CGGACTC30450.015.18883428
CACGAGA31950.014.93896621
AGACGGA31950.014.76525725
CCTATCT31800.014.54402634
GCCCACG33450.014.37967118
CGAGCCC34350.014.21834115
GTATCAA175400.014.1755992
CCGAGCC35950.014.151614
ATCTCGT31650.014.08688737
CTATCTC33700.014.053411535
Produced by FastQC (version 0.11.5)