FastQCFastQC Report
Thu 28 Apr 2016
ERR1041751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6467262
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT591500.9146065212759279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT474570.733803578701466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346620.5359609677170958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227210.3513233266257034No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT221290.3421695301659342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG43750.020.38171410
CCCACGA45350.019.58103619
ACGAGAC45600.019.39254422
ACACATC47750.019.294246
CACGAGA46100.019.26247421
TCTCCGA46100.019.22234311
TACACAT49200.019.1392275
GCCCACG46400.018.93857818
ATCTCGT45300.018.58167837
CCGAGCC48750.018.3671814
ACATCTC51950.018.339758
CCACGAG48650.018.32887820
TCCGAGC49350.018.1438713
CGAGCCC49150.018.14242215
CATCTCG47350.018.12882836
CGAGACT49200.018.0487823
CTCCGAG50950.017.53778312
GGTATCA219550.017.1728531
GACTCCT52950.016.63078326
CACATCT55500.016.57
Produced by FastQC (version 0.11.5)