FastQCFastQC Report
Thu 28 Apr 2016
ERR1041750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5939859
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2753154.635042683673131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1747482.94195535617933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1331472.241585195877545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050811.7690823974104437No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT660791.1124674844975277No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA435060.7324416286649229No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA278180.46832761518413146No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231510.3897567265485595No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220270.37083371844348495No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA217680.3664733455794153No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT215560.3629042372891343No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT210570.35450336447380315RNA PCR Primer, Index 17 (95% over 21bp)
AAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAA200400.3373817459303327No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186340.3137111503825259No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA173930.29281839855121145No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159970.2693161571680405No Hit
CAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150100.2526996011184777No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147960.24909682199526959No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACAAAAAAAA140160.23596519715366981No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA139770.23530861591158983No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC130990.22052712025655827No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACAAAAAA127770.21510611615528247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127190.21412966200039427No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACAC126680.21327105576075123No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC126070.21224409535647226No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG122210.20574562460152673No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA116720.1965029809630161No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103460.17417921873229653No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATAC99790.16800062089015916No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90940.15310127731988252No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90430.15224267108023945No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCTGTCT90330.15207431691560355No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA89910.15136722942413278No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA88240.14855571487471336No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87700.14764660238567953No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA86890.14628293365212877No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACAC84550.14234344619964884No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA83400.14040737330633604No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACA83170.1400201587276735No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA78760.13259574006723054No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78550.13224219632149517No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATAC71150.11978398813843898No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACAAAAAAA69240.1165684235938934No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCT66140.11134944449018067No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACA66110.1112989382407899No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA64400.10842008202551609No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA63850.10749413412001867No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63210.10641666746634894No Hit
GCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59770.10062528420287417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT30450.026.48932637
GCCGTCT32400.024.89506136
TACACAT116250.023.0593535
ACGTTTA3550.021.36619826
ATACACA125700.021.1050134
ACACATC127950.020.8929276
ACATCTC138400.019.6228338
CACATCT136250.019.5387157
TCACGTT3850.019.22078124
ATCTCCG142800.018.69432810
GAGTACC5150.018.67961311
TGCGACG2200.017.6590922
CATCTCC153300.017.6311159
CGTTAAT1054.805588E-717.619051
TACCGAC3500.017.4428587
TGCCGTC47700.017.41404735
CGTCTTC9600.017.3437537
GAACAGT126300.016.8155171
ATACCGA3750.016.7733336
CGGTCGC2000.016.65000214
Produced by FastQC (version 0.11.5)