FastQCFastQC Report
Thu 28 Apr 2016
ERR1041749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489364
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT585492.351966205022648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT401601.613263468098679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT284011.1408938186621163No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT265011.0645691027909137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239700.9628965470698541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88820.3567979612463264No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA83590.33578857893020064No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.23142457270210384No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.2305809837372116No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56100.22535876633549773No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.20547416930589502No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49930.20057331912890203No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41690.1674724949826542No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG36710.14746738524378114No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35580.1429280731945991No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30400.1221195453939239No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA28970.11637510625203866No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT28890.11605373902731783TruSeq Adapter, Index 11 (95% over 21bp)
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG28190.11324177581101037No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC26230.10536827880534949No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA25980.10436400622809681No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC25950.1042434935188265No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.1012306757870685No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA24930.10014606140363563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT41850.024.5782555
CCGTCTT5100.023.21568737
ATCTCCG43850.023.16191510
TAACCGG400.001931816823.12522
ACACATC45200.022.7975676
ATCTCGT41900.022.78281837
ACATCTC45850.022.4743738
TCGCTAA1005.347829E-1022.19999914
CGCGTAT502.7033294E-422.19999924
GAGACCG45550.021.85071624
CCCACGA45900.021.84531619
CGAGCCC46500.021.84193415
GACCGTA45400.021.75991226
GCCCACG46450.021.70613518
GTACTAG45400.021.67841330
ACGAGAC46200.021.6634222
ATACACA47600.021.648114
TCTCCGA46950.021.63258711
GCCGTCT5500.021.52727336
ACTAGAT46250.021.5232
Produced by FastQC (version 0.11.5)