FastQCFastQC Report
Thu 28 Apr 2016
ERR1041748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7091956
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT347220.48959694617394695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236850.33396992310724993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT229620.3237752744094859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187730.26470835408454313No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC121070.17071453912009607No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG91430.12892070960395127No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA78010.1099978623668844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75310.10619073214780239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA5550.014.66666716
ACGGACC10150.013.66995058
GTATTAG20650.013.5278451
TAAGACT11900.013.2142854
AAGACGG11500.013.1913035
GACGGAC10200.012.8774517
TACTCCG7050.012.8581565
TAACGCC7100.012.7676064
TAACGAA7050.012.59574513
TACCGTC8600.012.4767447
ATAACGA6750.012.33333312
ATTAGAG12800.012.2851563
ACCGTCG7850.012.2547778
CGATCGC5150.012.21359319
TTAACGG4250.012.18823435
CCGTCGT8100.012.10493859
TAACGGC4300.012.04651236
CGTCGTA7750.011.93548510
TCGTTTA7450.011.91946330
ACGAACG7000.011.89285715
Produced by FastQC (version 0.11.5)