FastQCFastQC Report
Thu 28 Apr 2016
ERR1041747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8928348
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT632780.7087313352929343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT396360.4439343090121487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383780.4298443564251752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT332390.37228611608776896No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA122450.1371474319773378No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109920.12311348079174333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5650.016.04424935
TCTATAC12550.014.8884473
AAGACGG15350.014.8241045
ACGGACC14000.014.5357158
TAACGGC6250.014.50436
GACGGAC13900.014.3741017
GTATTAG29350.013.2367971
ATTAGAG15950.013.1065833
GCGCGTA1000.001834168912.95000111
CGCAAGA17300.012.6184972
TATACTG13950.012.465955
TAGAGTG11800.012.0720345
AGACGGA17100.012.0087726
TCGGTAC1552.7245216E-511.9354843
CTACACT24350.011.8521574
GTACATG422850.011.8039491
ATACCGT11950.011.7656916
CGTATTA5350.011.7570115
TACATGG422650.011.7307462
TTAGAGT11400.011.684214
Produced by FastQC (version 0.11.5)