FastQCFastQC Report
Thu 28 Apr 2016
ERR1041746.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041746.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8743001
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT536380.6134964413248952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315900.3613175842024952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT304090.3478096365309806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT256330.2931830843894448No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA110170.12600936451911648No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90550.10356855729514386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC14950.015.3444828
GACGGAC15000.015.0466667
AAGACGG17250.014.3710165
TACCGTC10000.013.8757
TCGTTTA9400.013.77659630
TATACTG15950.013.4545455
TAGAGTG11450.013.41048055
ACCGTCG9600.013.1041678
CGCAAGA19550.012.9641942
TAAACGC10600.012.74056628
ATTAGAG12900.012.6201553
CTAGCGG8950.012.60893929
CCGTCGT9850.012.5837569
AGACGGA18550.012.5660376
TTAGAGT11300.012.278764
ATACCGT12250.012.2326536
GTATTAG23850.012.1781971
TAATACT20400.012.151964
CGTCGTA10100.012.08910910
TAACGCC11200.012.0580364
Produced by FastQC (version 0.11.5)