FastQCFastQC Report
Thu 28 Apr 2016
ERR1041745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599039
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT610861.328234007148015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388290.8442850778173441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT351950.7652685702382607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291210.6331975006082793No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA147560.32084963837010294No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116600.2535312268497832No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.18482122025927591No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT75080.16325149667136982No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68140.14816138762902423No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA67280.1462914317534598No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA64830.1409642318753983No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA51450.11187119743929112No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48800.10610912410179604No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA47930.10421742455325994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT852.7259946E-517.4117646
ACGGACC6200.017.0080648
GACGGAC6250.016.8727
AGTACCG2300.016.0869565
AATCGTC2055.456968E-1215.34146328
AAGACGG7250.015.3103455
AGACGGA8000.014.1062516
ATTAGAC3050.013.950823
CGGACCA7450.013.906049
CGCAAGA8900.013.71912
GTATTAG10850.013.2995391
GTCCTAA6200.013.1290321
TACCGTC4850.012.9690727
GAACAGT65000.012.9215381
CGAACGA2305.7661964E-1012.86956516
GAACCGT2305.7661964E-1012.8695656
TCTATAC4900.012.8367343
AACAGTG64250.012.8132292
TAGTACC3400.012.5147064
GACTTTT15550.012.372994
Produced by FastQC (version 0.11.5)