FastQCFastQC Report
Thu 28 Apr 2016
ERR1041744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921287
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250070.8560268128396834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163720.5604379165758105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138910.4755095955994738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112220.38414575493609493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC63720.21812303960548896No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA61560.21072903826293002No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.17365633708704417No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG50030.17126013294825193No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA44410.15202203686251983No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.12354143909858908No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31480.10776072327025725No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.10296831499267275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC3250.015.9384627
CGAACGT1557.221388E-915.51612954
TCGAACG1557.221388E-915.51612953
ATACCGT3850.015.3766236
TACCCCG2300.015.2826095
TCTAGCG2350.014.95744628
CGTTATA1001.0942803E-414.82
TTAACGG1001.0942803E-414.835
TATACTG4500.014.85
TCTATAC4300.014.6279073
TGTACTA2800.014.5357145
CCGTCGT3200.014.4531259
ACCGTCG3100.014.3225818
GTATTAG7900.014.284811
TACGTCG1051.65701E-414.09523921
CTAGCGG2500.014.05999929
CGTCGTA3300.014.01515110
CGTATAC1203.3037835E-513.8750013
GTAAACG3600.013.87500127
GTATAGG5000.013.691
Produced by FastQC (version 0.11.5)