FastQCFastQC Report
Thu 28 Apr 2016
ERR1041743.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041743.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064964
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT495310.977914156941688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308280.6086519074962823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289260.5710998143323427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238020.4699342384269661No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA112410.2219364244247343No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92230.18209408793428739No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.1333079563842902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60560.11956649642524605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC55080.10874707105519407No Hit
GAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAAA55030.10864835367043083No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54300.1072070798528874No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA54260.1071281059450768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1001.09453234E-414.82
ACGGACC7050.014.4326238
AAGACGG7550.014.2119215
CGAACGT2353.6379788E-1214.1702124
GACGGAC7050.013.9078017
CGTTCGG1102.458952E-413.45454524
GCGCTTA1703.73866E-713.05882423
TATACTG8400.012.773815
GTGTAAG8300.012.704821
AACAGTG48300.012.6397512
TTAACGG2204.094545E-912.61363635
GAACAGT49450.012.6076851
AGACGGA8400.012.5535736
CGCGAAA1205.126788E-412.33333415
AATACTG12100.012.2314045
TCTAGCG5150.012.21359328
CGGACCA8200.012.1829269
TCGAACG2756.184564E-1112.1090913
TACCGTC5350.012.1028037
TAATACT13550.012.014764
Produced by FastQC (version 0.11.5)