FastQCFastQC Report
Thu 28 Apr 2016
ERR1041742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110464
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT571980.8044200772270277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376360.5293044167019199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT336590.47337276442156234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266970.3754607294263778No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA137780.19377075813899067No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122100.171718751406378No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120200.16904663324362518No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG97080.13653117433686465No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.12218611893682325No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA82230.11564646132798086No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74590.10490173355775376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT73400.10322814376108225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA2250.017.2666662
CTAACGC803.385653E-416.18753
TCGAACG3150.015.2698423
CGAACGT3100.014.9193554
ATACCGT9200.014.8804356
CGTCGTA6900.014.74637710
TAACGGC3650.014.6986336
TTAACGG3700.014.535
TACCGTC8300.014.4879527
ACCGTCG7450.014.4026848
AAGACGG12000.013.8755
ATTAGAG10800.013.7037043
TTAGACT7050.013.64539054
TAGACTA6550.013.5572515
CCGTCGT7700.013.4545459
ACGGACC10850.013.299548
TACGACG8950.013.2290515
CGACGGT8750.013.1085727
CTAGCGG6400.013.007812529
GTAAACG9350.012.86096227
Produced by FastQC (version 0.11.5)