FastQCFastQC Report
Thu 28 Apr 2016
ERR1041741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026358
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT337910.5607200899780597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240940.39981030001868456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT202520.3360570347795468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167290.2775971822450641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC99900.16577176463794552No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA98550.16353160565635164No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.13291609957456893No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA70040.11622276671913617No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68920.11436426445292497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT8600.014.1976746
ACCGTCG6650.014.187978
ACGGACC10200.014.14705858
TCGTTTA7900.014.05063230
TTAACGG3300.014.01515135
AAGACGG10500.013.9190475
GACGGAC9600.013.8750017
GGTTATC8100.013.7037042
TAACGGC3300.013.45454536
TACCGTC7600.013.3881587
TATACCG2102.046363E-913.2142855
TATATCG3550.013.028175
ATAACGC15850.012.6056783
GTATTAG18700.012.5641711
TAACGCC9300.012.5322584
CCTATAC9100.012.4010983
CGTTTAT9400.012.39893631
CCGTCGT7650.012.3333359
CGTCGTA7200.012.33333310
TTAATAC8300.012.2590363
Produced by FastQC (version 0.11.5)