FastQCFastQC Report
Thu 28 Apr 2016
ERR1041740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466967
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1279111.9779132938207353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT825091.2758531163062994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT653551.0105973944199809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT504220.7796854383206223No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA318670.492765774125645No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC310230.47971483386261293No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248780.3846934737721717No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG240760.3722919878824184No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA218920.3385203604719183No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179140.277007753402793No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT162520.2513079160601871No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152950.2365096342690476No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG144190.2229638716263745No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG143140.22134023569317735No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT127640.19737227667931506No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA118210.1827904796792685No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA107480.16619846676193029No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG106330.16442019883509534No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA101540.1570133263398437No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA99590.1539980024639062No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC91970.1422150445487042No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA84570.13077227701950545No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA82860.1281280699282987No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA75930.11741207276919768No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75730.11710280878192204No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75320.11646881760800697No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA74160.11467508648180824No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA68760.1063249588253659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA2450.019.6326542
CGTACGT500.007038317618.49999813
TCGAACG1901.8189894E-1216.5526313
CGAACGT2003.6379788E-1215.7249994
CGACGGT4050.015.5308647
TAACCGG2750.015.47272722
ACGGACC6750.015.3481488
GACGGAC6650.015.3007537
ACGACGG4300.015.0581396
TACGACG4400.014.7159095
TTAACGG5700.014.60526335
TAACGGC5750.014.47826136
TACGTAG4000.014.33749914
ATTAGAG9050.014.3093923
GTACGTA3750.014.30666613
GACTTTT29000.014.2896564
GATACAC7800.014.230773
TCGAATT2500.014.05999913
CTACCCG5400.014.04629510
TTGCGAT1601.7873208E-713.87516
Produced by FastQC (version 0.11.5)