FastQCFastQC Report
Thu 28 Apr 2016
ERR1041739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7096190
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1537192.166218773736329No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1044391.4717616072850361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT781421.1011824655202298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT590630.832319878695469No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA417660.5885693590504201No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327390.4613602510643035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC284380.40075026176018397No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226370.31900216876943827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA212020.29878004957589915No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208170.2933546029629985No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT205010.28890150911968254No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG203370.2865904097832781No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG169030.23819824441002846No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA160840.22665683979713058No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG158690.22362704493538083No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT133780.1885237007464569No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA124810.17588311474185445No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA116670.16441217047457862No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA114740.16169240113356603No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA111720.15743659625799197No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA103820.14630386164970216No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA100520.1416534788386444No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99580.14032882434094915No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC99120.13968058916122597No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA96090.13541069221652746No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95850.13507248255754145No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95690.13484700945155076No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89550.12619447900915842No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG88040.12406657657137139No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA85620.12065629584326236No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84200.1186552220275951No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA83570.11776742167275678No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80390.11328614369119203No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA74950.10562005808750893No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA73670.10381627323958349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG2100.020.26190424
CGTTATA2200.018.52
GATACAT10000.017.021
CGGTAAG2400.016.95833428
TTAACGG5300.016.4056635
GAGGACT38000.016.3578951
GACTTTT38200.016.2722514
CGTTAAT2200.015.9772731
TCGGTAA2550.015.96078427
TACCGTC4450.015.7977537
CGAACGT2250.015.6222224
TCGAACG2250.015.6222223
TTCGGTA2750.015.47272826
ACCGTCG4200.015.4166668
TAACGGC5650.015.3893836
CCGTCGT4100.015.3414649
CGTCGTA4000.015.26250110
GGACTTT42100.014.7648463
ATTAGAG10250.014.4390243
ATTAGAC4300.014.1976743
Produced by FastQC (version 0.11.5)