FastQCFastQC Report
Thu 28 Apr 2016
ERR1041737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6948756
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT532930.7669430326809575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376510.5418379922967507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317330.45667166900089745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT247990.35688402355759796No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC172960.24890786206912432No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA126030.18137059352781995No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG122550.17636250287101748No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122360.17608907263400816No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA122120.17574368707147006No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84630.12179158398999763No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79680.11466800676264931No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78810.11341598409844869No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG76250.10973187143137562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.10545772509496663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19850.015.0982371
TTAACGG3750.013.3235
TCGTTTA9550.012.7853430
ATTAGAG11550.012.653683
AAGACGG13150.012.5209135
TAGAGTG9100.012.4010995
ATACCGT10500.012.3333346
CTAGCGT1359.97404E-512.3333344
CGAACGA5550.012.33333316
TCTATAC7850.012.2547773
TATACTG10900.012.2201845
GAACAGT66650.012.1297831
GGTTATC7350.012.0816332
GACGGAC12300.012.0325217
AACAGTG67200.011.9203862
TAACGGC4350.011.90804736
ACGGACC12850.011.8054488
TAATACT16700.011.7425154
TATCTAG11400.011.6842111
ACCGTCG7850.011.54777058
Produced by FastQC (version 0.11.5)